Journal: Life sciences

Article Title: Effects of chitinase-1 inhibitor in obesity-induced and -aggravated asthma in a murine model

doi: 10.1016/j.lfs.2023.122163

Figure Lengend Snippet: TGF- β (A), IL-1 β (B), IL-6 (C), TNF- α (D), IL-13 (E), and IL-4 (F) levels in lung homogenates Bar and line graphs present mean ± standard error. Cht, chitin; CPX, chitinase-1 inhibitor; OVA, ovalbumin * P < 0.05 between them analyzed by one-way ANOVA followed by post-hoc Bonferroni test.

Article Snippet: The concentrations of chitinase-1 (Fine biotech, EM0939, Wuhan, China), hydroxyproline (Biovision, K555–100, CA, USA), collagen-1 (Fine biotech, EM6446), interleukin (IL)-1 β (DY401), TNF- α (DY410), IL-4 (DY404), IL-6 (DY406), IL-13 (DY413), and TGF- β (DY1679) in lung homogenates were assessed using an enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, San Diego, USA) according to the manufacturer’s instructions.

Techniques:

Journal: Life sciences

Article Title: Effects of chitinase-1 inhibitor in obesity-induced and -aggravated asthma in a murine model

doi: 10.1016/j.lfs.2023.122163

Figure Lengend Snippet: Pathologic findings (H&E, PAS, and MT; all × 200 magnification) (A), quantitative fibrosis area (B), level of hydroxyproline in lung homogenates (C), and level of collagen-1 in lung homogenates (D). TGF- β (E), TNF- α (F), IL-1 β (G), IL-6 (H), IL-4 (I), and IL-13 (J) levels in lung homogenates Bar and line graphs present mean ± standard error. Cht, chitin; CPX, chitinase-1 inhibitor; HFD, high-fat diet; OVA, ovalbumin; H&E, hematoxylin and eosin; PAS, periodic acid-Schiff; MT, Masson’s trichrome * P < 0.05 between them analyzed by one-way ANOVA followed by post-hoc Bonferroni test.

Article Snippet: The concentrations of chitinase-1 (Fine biotech, EM0939, Wuhan, China), hydroxyproline (Biovision, K555–100, CA, USA), collagen-1 (Fine biotech, EM6446), interleukin (IL)-1 β (DY401), TNF- α (DY410), IL-4 (DY404), IL-6 (DY406), IL-13 (DY413), and TGF- β (DY1679) in lung homogenates were assessed using an enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, San Diego, USA) according to the manufacturer’s instructions.

Techniques:

Journal: Advanced Science

Article Title: IL‐4/STAT6‐signaling Influences Local Inflammation and Regeneration Processes During Acute Pancreatitis and Promotes Fibrosis by a Direct Activation of Pancreatic Fibroblasts During Chronic Pancreatitis

doi: 10.1002/advs.202515585

Figure Lengend Snippet: CD206 M2‐like macrophages differentiate in the absence STAT6 but retain proinflammatory properties (A) Immunofluorescent labeling of CD206, CCR2 and CD3 in CP tissue. (B) Quantification of CD206 + , CCR2 + and CD3 + cells in the pancreas of CP mice ( n = 10). (C) Heat map illustrating different levels of inflammation related proteins between wild type and Stat6‐/‐ mice. The numbers in the boxes represents the change compared with untreated control mice, the amount of all red labeled proteins were significantly changed in Stat6‐/‐ compared to wild type mice after onset of CP ( n = 5). (D) Quantitative gene expression analysis by RT‐qPCR of Arg1 , Fizz , Ym1 and Il10 in CP tissue of wild type and Stat6‐/‐ mice ( n = 5). (E) Immunofluorescent labeling of MMP9 (red) and α‐amylase (green) in CP tissue and quantification of MMP9 + cells in the pancreas of CP mice. (F) Quantitative RT‐PCR of Nos2 , Il1b , Mmp9 and Il6 in CP tissue of wild type and Stat6‐/‐ mice ( n = 10). (G) Measurement of TGF‐β in serum of untreated control mice and CP mice ( n = 9). (H) Quantitative RT‐PCR of Tgfbi and Csf1 in CP tissue of wild type and Stat6‐/‐ mice ( n = 5). Data in B and D‐H are shown as mean +/‐ SEM. Differences were tested for statistical significance by unpaired students t‐test or Mann–Whitney for non‐normally distributed samples for independent samples, significance levels of p < 0.05 are marked by an asterisk.

Article Snippet: Recombinant Mouse IL‐4 Protein (#404‐ML, R&D Systems), Recombinant Mouse IL‐13 Protein (#413‐ML, R&D Systems), Lipopolysaccharide Escherichia coli O26:B6 (L8274, Sigma Aldrich), Recombinant Mouse IL‐6 Protein (406‐ML, R&D systems), Recombinant Mouse TGF‐β Protein (7666‐MB, R&D systems), Recombinant mouse IL‐10 (575804, BioLegend).

Techniques: Labeling, Control, Gene Expression, Quantitative RT-PCR, MANN-WHITNEY

Journal: Naunyn-Schmiedeberg's archives of pharmacology

Article Title: Alleviation of pulmonary fibrosis by the dual PPAR agonist saroglitazar and breast milk mesenchymal stem cells via modulating TGFß/SMAD pathway.

doi: 10.1007/s00210-024-03004-y

Figure Lengend Snippet: Fig. 3 Effect of BrMSCs and/or saroglitazar on lipid peroxidation parameters A MDA and B SOD, the pro-inflammatory cytokines C IL-1β, D IL-10, E TGF-ß, and F serum Beclin-1 in rats with BLM-induced lung fibrosis

Article Snippet: To localize alpha-smooth muscle actin (α-SMA) protein as a marker for myofibroblasts, anti-alpha-smooth muscle actin antibody (rabbit polyclonal antibody; No. ab5694; dilution 1/50; Abcam, Cambridge, UK) (Egger et al. 2013) and with a monoclonal rabbit anti-TGFβ1 antibody (Clone TB21, MCA797, Serotec, Oxford, UK) at 1:200 dilution for TGF-β1 detection (Attia et al. 2017).

Techniques:

Journal: Naunyn-Schmiedeberg's archives of pharmacology

Article Title: Alleviation of pulmonary fibrosis by the dual PPAR agonist saroglitazar and breast milk mesenchymal stem cells via modulating TGFß/SMAD pathway.

doi: 10.1007/s00210-024-03004-y

Figure Lengend Snippet: Fig. 4 Effect of BrMSCs and/or saroglitazar on the mRNA expression genes of A TGF-β, B SMAD-3, C SMAD-7, D PPAR-γ, E PPAR-α in rats’ lungs with BLM-induced pulmonary fibrosis

Article Snippet: To localize alpha-smooth muscle actin (α-SMA) protein as a marker for myofibroblasts, anti-alpha-smooth muscle actin antibody (rabbit polyclonal antibody; No. ab5694; dilution 1/50; Abcam, Cambridge, UK) (Egger et al. 2013) and with a monoclonal rabbit anti-TGFβ1 antibody (Clone TB21, MCA797, Serotec, Oxford, UK) at 1:200 dilution for TGF-β1 detection (Attia et al. 2017).

Techniques: Expressing

Journal: Naunyn-Schmiedeberg's archives of pharmacology

Article Title: Alleviation of pulmonary fibrosis by the dual PPAR agonist saroglitazar and breast milk mesenchymal stem cells via modulating TGFß/SMAD pathway.

doi: 10.1007/s00210-024-03004-y

Figure Lengend Snippet: Fig. 10 A photomicrograph of immune-stained sections of the study groups. A CN-G showing faint expression of TGF-β1 by some cells in the alveolar septa between the alveoli and alveolar sacs. B, C BLM-G sections showing [B] Numerous cytoplasmic reactions for TGF- β1 positive immune expressed cells (arrows) within the inter- alveolar septum. C Increased TGF-β1 positive immune expressed cells (arrows) within the inter-alveolar septum. D BrMSCs-G showing some positive immune expressed cells (arrows) for TGF-β1 within the inter-alveolar septum. E Saro-G showing few positive immune expressed cells (arrows) for TGF-β1 within the inter-alveo- lar septum. F BrMSCs + Saro-G showing faint weak positive TGF-β1 immune expressed cells (arrows) within the inter- alveolar septum. (TGF-β1 X 400) (scale bar = 40 μm). G Mean values of % area of TGF-ß1 among different studied groups, one way ANOVA was used for statistical analysis. “a” means value is statistical significance when comparing CN-G with other groups, “b” means the value is statistical significance when comparing BLM-G with other groups, “c” means the value is statistical significance when comparing BrMSCs + Saro-G with other groups. The same letters mean no statistical significance dif- ference

Article Snippet: To localize alpha-smooth muscle actin (α-SMA) protein as a marker for myofibroblasts, anti-alpha-smooth muscle actin antibody (rabbit polyclonal antibody; No. ab5694; dilution 1/50; Abcam, Cambridge, UK) (Egger et al. 2013) and with a monoclonal rabbit anti-TGFβ1 antibody (Clone TB21, MCA797, Serotec, Oxford, UK) at 1:200 dilution for TGF-β1 detection (Attia et al. 2017).

Techniques: Staining, Expressing